Early heart development gene networks and morphogenesis in the Ciona intestinalis chordate tadpole. Jennifer Jean Beh

ISBN: 9780549836582

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NOOKstudy eTextbook

108 pages


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Early heart development gene networks and morphogenesis in the Ciona intestinalis chordate tadpole.  by  Jennifer Jean Beh

Early heart development gene networks and morphogenesis in the Ciona intestinalis chordate tadpole. by Jennifer Jean Beh
| NOOKstudy eTextbook | PDF, EPUB, FB2, DjVu, AUDIO, mp3, ZIP | 108 pages | ISBN: 9780549836582 | 3.67 Mb

Heart development requires precise coordination of morphogenetic movements with progressive cell fate specification and differentiation. In ascidian embryos, FGF/MAPK mediated activation of the transcription factor Etsl/2 is required for heart tissueMoreHeart development requires precise coordination of morphogenetic movements with progressive cell fate specification and differentiation. In ascidian embryos, FGF/MAPK mediated activation of the transcription factor Etsl/2 is required for heart tissue specification and cell migration.

I found that the forkhead transcription factor FoxF and the sole cardiac GATA factor, GATA-a, are among the first genes to be activated in heart precursors in response to FGF signaling. I identified the FoxF minimal heart enhancer and used a cis/trans complementation test to show that Etsl/2 can interact with the FoxF enhancer in vivo. Next, I found that FoxF function is required downstream and in parallel to the FGF/MAPKIEts cascade for cell migration. In addition, I demonstrated that targeted expression of a dominant-negative form of FoxF inhibits cell migration but not heart differentiation, resulting in a striking phenotype: a beating heart at an ectopic location within the body cavity of juveniles.

Taken together, my results indicate that FoxF is a direct target of FGF signaling specifically involved in the regulation of heart cell migration. I also discovered that GATA-a is specifically involved in the migration of heart precursor cells in the Ciona embryo. A dominant negative form of GATA-a blocked heart cell migration and produced mis-localized beating heart tissue in the juvenile, indicating embryonic GATA-a is not necessary for heart tissue differentiation. GATA-a is also a downstream target of the FGF/MAPK/Ets cascade and regulates both NK-4 and FoxF for the proper genetic expression in heart precursor cells.



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